Purpose: In the realm of laparoscopic cholecystectomy, the significance of Rouviere's Sulcus as a pivotal physiological landmark cannot be overstated. Its identification plays a crucial role in facilitating the meticulous dissection of Calot's triangle, ensuring the secure ligation of the cystic artery and duct, and ultimately preventing common bile duct injuries. Understanding the anatomical variations of Rouviere's Sulcus is paramount for surgeons aiming to enhance the precision and safety of this common surgical procedure. Materials and methods: This observational study was designed to meticulously examine 49 patients presenting with confirmed gallstones, identified through ultrasound of the abdomen, in the general surgery outpatient department. The individuals included in the study were aged 19 years and above, representing both genders. The study methodology involved a comprehensive observation during laparoscopic cholecystectomy procedures. Results: Among the 49 patients subjected to laparoscopic cholecystectomy, the visualization of Rouviere's Sulcus was achieved in 46 cases (93.9%). The observed variations in Rouviere's Sulcus were diverse, revealing distinct anatomical configurations: Open type: 23 cases (46.9%), closed type: 7 cases (14.3%), slit type: 9 cases (18.4%), scar type (oblique): 4 cases (8.2%), scar type (transverse): 3 cases (6.1%) and absent: 3 cases (6.1%). Conclusion: These findings underscore not only the high prevalence of Rouviere's Sulcus but also the diverse nature of its anatomical presentations. The recognition of such variations emphasizes the need for a nuanced and individualized surgical approach, ensuring the utmost safety and efficacy in laparoscopic cholecystectomy.