World Journal of Laparoscopic Surgery

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VOLUME 3 , ISSUE 1 ( January-April, 2010 ) > List of Articles


Effects of CO2 Pneumoperitoneum and Enteric Disturbance on β-endorphin in SD Rats

Wu Gang, Cai Duan, Lu Lei, Ma Bao-Jin, Zhang Yan-Ling

Citation Information : Gang W, Duan C, Lei L, Bao-Jin M, Yan-Ling Z. Effects of CO2 Pneumoperitoneum and Enteric Disturbance on β-endorphin in SD Rats. World J Lap Surg 2010; 3 (1):49-53.

DOI: 10.5005/jp-journals-10007-1082

Published Online: 01-08-2012

Copyright Statement:  Copyright © 2010; The Author(s).



To study the effects of enteric disturbance and CO2 pneumoperitoneum on serum β-endorphin (β-EP) in SD rats, and discuss their influences on perioperative stress responses.


120 SPF-grade male SD rats were anesthetized intraperitoneally and equally randomized to four groups: group A, CO2 pneumoperitoneum at 1.0 kPa; group B, a 5 cm abdominal incision without enteric disturbance; group C, a 5 cm abdominal incision with enteric disturbance; and group D, control Group. Serum β-EP was measured at 10, 20 and 40 minutes after initiation of surgery.


The serum β-EP concentration of group A was 2.74 ± 0.67 ng/ml, 1.57 ± 0.64 ng/ml and 1.64 ± 0.74 ng/ml at 10, 20 and 40 minutes of CO2 pneumoperitoneum respectively, which was significantly higher than that of the control group (P < 0.01). The serum β-EP concentration of group B was 2.53 ± 0.86 ng/ml, 1.46 ± 0.11 ng/ml and 1.34 ± 0.14 ng/ml at 10, 20 and 40 minutes after the 5 cm abdominal incision was made, which was very significantly higher than that of the control group (P < 0.01). The serum –EP concentration of group C was 3.77 ± 0.51 ng/ml, 2.99 ± 0.70 ng/ml and 2.67 ± 0.54 ng/ml at 10, 20 and 40 minutes after the 5 cm abdominal incision was made with enteric disturbance. There was a very significant difference in the concentration of serum β-EP at 10, 20 and 40 minutes between Group C and Group B (P < 0.01).


Both enteric disturbance and CO2 pneumoperitoneum are important stimulating factors inducing stress responses in rats. Enteric disturbance may accentuate the severity of stress responses in laparotomy.

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