Aims and objectives: This retrospective type of study was done to know the bacterial cause of cholecystitis and to isolate different bacteria present in bile of cholecystectomy patients. Antibiotic sensitivity was also done to know the antibiotic-resistance pattern among the organism isolated. Materials and methods: In this study, 126 patients’ bile was sent to the Microbiology Department for culture and antibiotic-sensitivity testing during the period of October 2017–November 2018. Cultures were placed in blood agar and MacConkey agar. Organisms were isolated on the basis of growth characteristics and biochemical findings. Antibiotic sensitivity was done using the Kirby–Bauer disk-diffusion method. Results: This study included 126 post-cholecystectomy patients, out of which the male-to-female ratio was 1:2.71. While the female was 92 (83%) and the male was 34 (17%). In this study, we have included all the age-groups of patients, but most of the patients were middle-aged, that is, between 41 and 60 years 78 (62%). In the microbiological analysis, only 68 (54%) samples were culture-positive. In our study, Escherichia coli 43 (63.2%) was isolated among maximum samples and the second most common was Klebsiella spp. 17 (25%). Conclusion: Therefore, it is important to know about common bacteria causing gallbladder infection and their antibiotic-resistance pattern. This study may be helpful in designing the antibiotic prophylaxis among these patients.
van Leeuwen PA, Keeman JN, Butzelaar RM, et al. Correlation between a positive gallbladder culture and subsequent wound infection after biliary surgery: A retrospective study of 840 patients. Neth J Surg 1985;37(6):179–182. PMID: 3911113.
Md Khalid Anjum, Amber Prasad, Makbul Ansari, et al. Bacteriological profile of bile in cholecystectomy patients at Rims, Ranchi, India. Int J Curr Microbiol Appl Sci 2020;9(6):156–160. DOI: 10.20546/ijcmas.2020.906.020.
José Eleuterio González, Rubén Cueto-Ramos, Marco Hernández-Guedea, et al. Incidence of bacteria from cultures of bile and gallbladder wall of laparoscopic cholecystectomy patients in the University Hospital. Cirugía y Cirujanos 2017;85(6):515–521. DOI: 10.1016/j.circen.2016.10.009.
Kumar M, Oraon V, Sherwal BL, et al. Bacterial profile of bile and gall stone in symptomatic cholelithiasis patients. Int J Med Res Prof 2017;3(3):122–126. Doi: 10.21276/ijmrp.2017.3.3.026.
Ahmad F, Islahi S, Hingora OM, et al. Cholelithiasis – A clinical and microbiological analysis. Int J Sci Stud 2014;2(4):40–45.
Sabharwal ED, Chopra R, Chawla LS. Gall bladder disease pattern in Punjab. J Indian Med Assoc 1984;82(5):164–166. PMID: 6481174.
Manan F, Khan MA, Faraz A, et al. Frequency of common bacteria and their antibiotic sensitivity in patients with symptomatic cholelithiasis. J Postgraduate Med Instit 2014;28(2):177–183.
Chhutani PN, Sachdeva Y, Chitkara NL, et al. Gall bladder disease in Punjab: Incidence and symptomatology. J Assoc Physicians India 1965;13:140–145. PMID: 14263397.
Capoor MR, Nair D, Khanna G, et al. Microflora of bile aspirates in patients with acute cholecystitis with or without cholelithiasis: A tropical experience. Braz J Infect Dis 2008;12(3):222–225. DOI: 10.1590/s1413-86702008000300012.
Bhansali SK. Preoperative complications of gallstones and their relevance to treatment and prognosis: Experience with 451 cases. Am J Gast 1985;80(8):648–654. PMID: 4025282.
Flores C, Maguilnik I, Hadlich E, et al. Microbiology of choledochal bile in patients with choledocholithiasis admitted to a tertiary hospital. J Gastroenterol Hepatol 2003;18(3):333–336. DOI: 10.1046/j.1440-1746.2003.02971.x.
Sharma K, Jad B, Mahajan B, et al. Bacteriological analysis of bile culture from a tertiary care hospital. Int J Health Sci Res 2018;8(5):88–91.
Pratik MP, Nimish JS, Pokhraj PS, et al. Bacteriological analysis of bile in cholecystectomy patients. Int J Res Med Sci 2015;3(11):3091–3096. DOI: 10.18203/2320-6012.ijrms20151142.
Fuks D, Cossé C, Régimbeau JM. Antibiotic therapy in acute calculous cholecystitis. J Visc Surg 2013;150(1):3–8. DOI: 10.1016/j.jviscsurg.2013.01.004.