Context: This study was undertaken to reduce the incidence of port site infection (PSI). Aim: The study aimed to evaluate the impact of povidone iodine (PI)-dipped ports on PSI and compare it to non-PI-dipped ports. Materials and methods: A total of 164 patients undergoing elective laparoscopic surgery were enrolled in the study. All patients underwent routine preoperative workup. They were randomized into control and intervention groups. For patients in the intervention group, ports were dipped in 10% PI solution 5 minutes prior to usage. In the control group, conventional techniques were used. Patients were evaluated for infections on days 1, 3, 7, and 30. Statistical analysis: Data was compiled in Microsoft Excel and processed using Statistical Package for the Social Sciences (SPSS) software. Quantitative parameters were compared using the t-test while qualitative were compared using the Chi-square test. Results: The two groups were equally matched with respect to demographic and laboratory factors with no statistically significant difference between the two. Port sites were evaluated on days 1, 3, 7, and 30 using the Southampton scoring system. In the intervention group, infection was found to be 3.6% on day 1; 6.1% on day 3; and 1.2% on days 7–30. No statistically significant difference was found when compared to the infection rate in the control group (3.6, 2.4, and 1.2%). Conclusion: Ports dipped in PI have no significant impact on the incidence of PSI in elective laparoscopic surgeries.