Background: Total mesorectal excision (TME) by conventional laparotomy has been considered the standard of care for patients with rectal cancer. Over the past two decades, numerous prospective randomized studies have reported the feasibility, safety, and advantages of laparoscopic colorectal surgery. The benefits of laparoscopic surgery are advantageous only when the quality of the TME, as demonstrated by standardized pathological measures, is at least similar, if not superior, to that of open TME. However, in most of the studies, the impact of laparoscopic TME on pathological outcomes has been inconsistently reported. Therefore, we aimed to assess the quality of TME in laparoscopic resection of rectal cancer. Materials and methods: We reviewed retrospectively the medical records of patients who underwent laparoscopic resection for rectal cancer post-chemoradiation during the period from 2017 to 2021 at our institute. Patient data were collected from the cancer registry. The quality of pathological outcomes was analyzed by the completeness of TME, circumferential margins, lymph node harvest, and distal resection margins. Data analysis was done using MS Excel, and SPSS 28.0 (Trail version). Using this software, frequencies, percentage, range, mean, and standard deviation. Chi-square test, t-test, and p-values were calculated. Results: A total of 64 patients were included in the study. And 35 patients underwent low anterior resection (55%), 22 patients underwent abdominal perineal resection (34%), and 7 patients underwent anterior resection (11%). The mesorectum excision was complete in 58 patients (90.48%) and near complete in 6 patients (9.52%). The average number of lymph nodes harvested was 10. The multivariable analysis between patients with lymph nodes retrieved less than 12 and greater than 12 shows that the lymph node retrieved is less than 12 if the interval between radiotherapy and surgery is less than 6 weeks which is statistically significant (p-value –0.04). And there was no statistically significant association between the number of nodes retrieved and survival rate. Positive circumferential margins were seen in 2 patients (4%) and the rest 62 patients (96%) showed negative margins. In all the patients, distal resected margins were free of tumors. In a follow-up of 2 years, distant metastasis was seen in 5 patients. No one had local recurrence. Conclusions: Our study has shown that optimal pathological outcomes can be achieved with laparoscopic mesorectal excision in rectal cancer patients. Among patients who received preoperative chemoradiation, the number of lymph nodes retrieved was not associated with overall survival.