Effects of CO2 Pneumoperitoneum and Enteric Disturbance on β-endorphin in SD Rats
Wu Gang, Cai Duan, Lu Lei, Ma Bao-Jin, Zhang Yan-Ling
Citation Information :
Gang W, Duan C, Lei L, Bao-Jin M, Yan-Ling Z. Effects of CO2 Pneumoperitoneum and Enteric Disturbance on β-endorphin in SD Rats. World J Lap Surg 2010; 3 (1):49-53.
To study the effects of enteric disturbance and CO2 pneumoperitoneum on serum β-endorphin (β-EP) in SD rats, and discuss their influences on perioperative stress responses.
Methods
120 SPF-grade male SD rats were anesthetized intraperitoneally and equally randomized to four groups: group A, CO2 pneumoperitoneum at 1.0 kPa; group B, a 5 cm abdominal incision without enteric disturbance; group C, a 5 cm abdominal incision with enteric disturbance; and group D, control Group. Serum β-EP was measured at 10, 20 and 40 minutes after initiation of surgery.
Results
The serum β-EP concentration of group A was 2.74 ± 0.67 ng/ml, 1.57 ± 0.64 ng/ml and 1.64 ± 0.74 ng/ml at 10, 20 and 40 minutes of CO2 pneumoperitoneum respectively, which was significantly higher than that of the control group (P < 0.01). The serum β-EP concentration of group B was 2.53 ± 0.86 ng/ml, 1.46 ± 0.11 ng/ml and 1.34 ± 0.14 ng/ml at 10, 20 and 40 minutes after the 5 cm abdominal incision was made, which was very significantly higher than that of the control group (P < 0.01). The serum –EP concentration of group C was 3.77 ± 0.51 ng/ml, 2.99 ± 0.70 ng/ml and 2.67 ± 0.54 ng/ml at 10, 20 and 40 minutes after the 5 cm abdominal incision was made with enteric disturbance. There was a very significant difference in the concentration of serum β-EP at 10, 20 and 40 minutes between Group C and Group B (P < 0.01).
Conclusion
Both enteric disturbance and CO2 pneumoperitoneum are important stimulating factors inducing stress responses in rats. Enteric disturbance may accentuate the severity of stress responses in laparotomy.
Stress responses to endoscopic surgery. Current Opinion in Obstetrics and Gynecology 2000,12:303-07.
Neuroendocrine and behavioral effects of CRH blockade stress in male rats. Physiol Behav 1999;66(3):523-28.
Direct effects of corticotrophin-releasing hormone on stimulated growth hormone secretion. Clin Endocrinol 1998;48(2):217-22.
Neuroendocrine mechanisms of impact experimental stress on plasma glucose level in STZ KM mice. ZhonghuaYiXueZaZhi 1999;79(5):332-34.
Differences in the Responses of the Pituitary β-Endorphin and Cardiovascular System to Ethanol and Stress as a Function of Family History. Alcohol Clin Exp Res 2002;26(8):1171-80.
The stress response and the regulation of inflammatory disease. Ann Intern Med 1992;117(10):854-66.
A two-step mechanism by which corticotrophin-releasing hormone releases hypothalmic beta-endorphin: The role of vasopressin and G-proteins. Endocrinolgy 1989;125(3):1365-72.
Corticotrophinreleasing hormone-related behaviour and neuroendocrine response to stress in Lewis and Fischer rats. Brain Res 1992;570(1-2):54-60.
Abnormal pituitary-adrenal responses to corticotrophin-releasing hormone in patients with seasonal affective disorder: Clinical and pathophysiological implications. J Clin Endocrinol Metab 1991;72(6):1382-87.
β-endorphin and adrenocorticotropin are secreted concomitantly by the pituitary gland. Science 1977;197(4311):1367-69.
Fentanyl and the β-endorphin, ACTH and glucoregulatory hormonal response to surgery. Br J Anaesth 1987;59(6):713-20.
Surgical stress and neuroendocrine responses in infants and children. J Pediatr Endocrinol Metab 1995;8(3):187-94.
Local increases of subcutaneous beta-endorphin immunoreactivity at the site of thermal injury. Immunopharmacology 1993;25(3):205-13.
Surgical stress in humans is accompanied by an increase in plasma beta-endorphin immunoreactivity. Life Sci 1981;29(12):1249-54.
Stress hyperglycemia in minimally invasive surgery. Surg Laparosc Endosc 1998;8(6):435-37.
Beta-endorphin (1 to 31) in the plasma of male volunteers undergoing physical exercise. Psychoneuroendocrinol 2000;25(6):551-62.
Beta-endorphin immunoreactive material and authentic beta-endorphin in the plasma of males undergoing anaerobic exercise on a rowing ergometer. Int J Sports Med 2000;21(7):513-17.
Proopiomelanocortin and exercise. In: Kraemer W, Rogol AD. The Endocrine System in Sports and Exercise. London, UK: Blackwell Publishing 2005:134-55.
Effects of acupuncture and transcutaneous stimulation analgesia on plasma hormone levels during and after major abdominal surgery. Eur J Anaesthesiol 1993;10(3):197-208.
Relationship Between Release of (beta)-Endorphin, Cortisol, and Trauma Severity in Children With Blunt Torso and Extremity Trauma. Journal of Trauma-Injury Infection and Critical Care 2007,62(2):320-24.
Plasma Levels of Corticotroph-type Pro-opiomelanocortin Derivatives Such as β-Lipotropin, β-Endorphin (1-31), or Adrenocorticotropic Hormone Are Correlated With Severity of Postoperative Pain. Clin J Pain 2006;22(2):113-21.
Endocrine and metabolic changes during surgery: Anaesthetic implications. Br J Anaesth 1981;53(2): 153-60.
Beta-endorphin and ACTH levels in the perioperative period. Gen Pharmacol 1989;20(4):399-402.
The pattern of endocrine response to surgical stress during different types of anesthesia and surgery in man. Acta Anesthesiol Belg 1986;37(1):23-31.
Impact of laparoscopic surgery on stress responses, immunofunction, and risk of infectious complications. New Horizons 1998;6(Suppl. 2):S80-88.
A prospective randomized comparison of the metabolic and stress hormonal responses of laparoscopic and open cholecystectomy. J Am Coll Surg 1996;183(3):249-56.
Evaluation of stress-related hormones after surgery by laparoscopy or laparotomy. J Am Assoc Gynecol Laparosc 1996;3(2):229-34.
Placebo and nocebo responses, cortisol, and circulating beta-endorphin. Psychosomatic Medicine 2003;5(65):786-90.
Operative stress response is reduced after laparoscopic compared to open cholecystectomy. Dig Dis Sci 2000;45(9):1703-13.
